Insertion junction: LMJ.RY0402.212523_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.212523
Chromosome:chromosome 13
Location:2468766
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g580000 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGCCAGCTGTCGGCGCGCGCTTTCGGC
Internal bar code:CAGTCCGTAGATGGTTCAGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:804
LEAP-Seq percent confirming:99.1945
LEAP-Seq n confirming:4064
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCACCACGCTCGTTATTT
Suggested primer 2:CTTCTTTGCCTACTCCGCAC