| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213456 |
| Chromosome: | chromosome 17 |
| Location: | 2363578 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g714900 | CGL11 | Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACGATGTACCGGCCCCACCCTGCTTTG |
| Internal bar code: | ACACGCAATTCTCTCGTCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 98.5437 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGTTGCTGAAACGAGTG |
| Suggested primer 2: | AACGTGACTACCCAGCATCC |