| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213494 |
| Chromosome: | chromosome 11 |
| Location: | 1326636 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467712 | SAGA1 | StArch Granules Abnormal 1; (1 of 3) IPR000104//IPR002044//IPR013784 - Antifreeze protein, type I // Carbohydrate binding module family 20 // Carbohydrate-binding-like fold | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGAAGGCTTGGGAGGGGGTTGCGTGAT |
| Internal bar code: | GCATCCGTCATTGAGATGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 82.0309 |
| LEAP-Seq n confirming: | 5049 |
| LEAP-Seq n nonconfirming: | 1106 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCACACAGCTCAAGGAG |
| Suggested primer 2: | GTGTTTATGTGAGGGGTGGG |