| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214073 |
| Chromosome: | chromosome 6 |
| Location: | 6173005 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g290400 | HDA5 | (1 of 1) PTHR10625:SF104 - HISTONE DEACETYLASE 6; RPD3/HDA1 type histone deacetylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTTGCGTAATCCACAGTAGTCCAAGTC |
| Internal bar code: | CTCATAGGCCGCACGAAATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 99.7802 |
| LEAP-Seq n confirming: | 2724 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATTGAAGCTGTGGTGCTA |
| Suggested primer 2: | CACTGCTAACTTCATGCGGA |