| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.214290 |
| Chromosome: | chromosome 4 |
| Location: | 2658649 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223050 | CAH2 | (1 of 2) PTHR18952:SF104 - CARBONIC ANHYDRASE-RELATED PROTEIN; Carbonic anhydrase, alpha type%252C periplasmic | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCTAGAGGCCTGCAAGGGCGGCTGCTT |
| Internal bar code: | ATGAATACTGGGTAGAAGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 97.5469 |
| LEAP-Seq n confirming: | 676 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACTGAAGCATTTTGCCTG |
| Suggested primer 2: | GTACTCACAAGCATCGGGGT |