| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214392 |
| Chromosome: | chromosome 14 |
| Location: | 522186 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611450 | PLP7,PLAP7 | Plastid lipid associated protein 7; (1 of 17) PF04755 - PAP_fibrillin (PAP_fibrillin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATGCACACACAAGGCAGAATCTCTCAAC |
| Internal bar code: | TTGGGAGTAGCGTCAGGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 98.3263 |
| LEAP-Seq n confirming: | 9341 |
| LEAP-Seq n nonconfirming: | 159 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTGCAAATGGAACGTATG |
| Suggested primer 2: | CACTCTTAGAGGAGCCCGTG |