| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214478 |
| Chromosome: | chromosome 10 |
| Location: | 2590233 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437150 | PPR6,CYCR5 | PentatricoPeptide Repeat protein 6; (1 of 2) K17710 - pentatricopeptide repeat domain-containing protein 1 (PTCD1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAGGTAGTGGCCCAGGCGCTCCAGGTA |
| Internal bar code: | GGTCAGGGACGAGTGGGGCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 79.4948 |
| LEAP-Seq n confirming: | 2675 |
| LEAP-Seq n nonconfirming: | 690 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTGCTGCTGAATGACTG |
| Suggested primer 2: | GGAACAAGCTGCTGAAGGAC |