| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.214947 |
| Chromosome: | chromosome 10 |
| Location: | 2509580 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436350 | SHK5,SHKF1 | (1 of 1) 2.7.1.71 - Shikimate kinase; Shikimate kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTCCGCACCCTCGATGGTGAAGTTCAT |
| Internal bar code: | CACATCCCGGAGAAGGGCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 99.7615 |
| LEAP-Seq n confirming: | 12546 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGGTCAGTTGTGTGGTC |
| Suggested primer 2: | ACTACTGCCTTGCCGTGACT |