| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.215234 |
| Chromosome: | chromosome 1 |
| Location: | 5923849 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042200 | PBA1,PBAS1 | Photosystem II Associated Subunit 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTTCATTGCTCCTCCTTCAGCCCGCGC |
| Internal bar code: | CACTTTTATTGTGTATCCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 997 |
| LEAP-Seq percent confirming: | 99.6287 |
| LEAP-Seq n confirming: | 12342 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGTAAACCTGGCCGTTTC |
| Suggested primer 2: | ACAACTCCCAACCACCAGAG |