| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.216061 |
| Chromosome: | chromosome 5 |
| Location: | 2499847 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235700 | (1 of 2) K12382 - saposin (PSAP, SGP1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTCATCCGCCCACGCACCCCGCACCTG |
| Internal bar code: | GCGGGGGATGCATCGATATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 44.5123 |
| LEAP-Seq n confirming: | 16259 |
| LEAP-Seq n nonconfirming: | 20268 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTCTTTTCCTGTGCGTC |
| Suggested primer 2: | ATCCTTCCATACAAGCACGG |