Insertion junction: LMJ.RY0402.217185_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.217185
Chromosome:chromosome 6
Location:2752835
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g270850 SRH12 (1 of 1) K10841 - DNA excision repair protein ERCC-6 (ERCC6, CSB, RAD26); SNF2-related DNA/RNA helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAGTCAGTCGCGGAGCAATGTGCACACA
Internal bar code:TGGTGCTCGGGAAGCTGTGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:56
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACGTGTTCATCTGTTTGCC
Suggested primer 2:ACACACACACACACACACCG