| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217239 |
| Chromosome: | chromosome 12 |
| Location: | 5860911 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533750 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCCGCAGACCGCAGCTGTCGCGCAGTCT |
| Internal bar code: | GTAGTGTGTCGGACAGAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 99.5922 |
| LEAP-Seq n confirming: | 6838 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTAGTATGCGGAGCCAGG |
| Suggested primer 2: | TAGGGTGCCAGTACTTTGGG |