| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217309 |
| Chromosome: | chromosome 2 |
| Location: | 5225578 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106000 | (1 of 8) IPR002867 - IBR domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGATCGCGCGCGGTCCGTTCCCATCAC |
| Internal bar code: | CGACAGTCTGGGACCAGTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 115 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTATGGCCACCTGGTTCTG |
| Suggested primer 2: | TTAAGGCCTGAAGTCGCTGT |