| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.217364 |
| Chromosome: | chromosome 1 |
| Location: | 4233001 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028350 | DEG8 | (1 of 2) PTHR22939:SF99 - PROTEASE DO-LIKE 8, CHLOROPLASTIC; Deg protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCCCGACCGAGGCCGTGCCCCAGGGAC |
| Internal bar code: | ACGAATGCCGAAACGTCGTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 713 |
| LEAP-Seq percent confirming: | 89.2304 |
| LEAP-Seq n confirming: | 3339 |
| LEAP-Seq n nonconfirming: | 403 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCAGGGTGCTAACGAAG |
| Suggested primer 2: | AAGCATACGTGATTGGGCTC |