| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217571 |
| Chromosome: | chromosome 2 |
| Location: | 3707263 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095200 | (1 of 3) PTHR19211:SF15 - ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTTGCGGGGCGTATGCATACAGTATAAA |
| Internal bar code: | CAGGGTTAGCCCGCAGGGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 986 |
| LEAP-Seq percent confirming: | 99.2151 |
| LEAP-Seq n confirming: | 17697 |
| LEAP-Seq n nonconfirming: | 140 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAATGTGCTCTGTCGATG |
| Suggested primer 2: | GCACGTGTGCTTGACTGTTT |