| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217703 |
| Chromosome: | chromosome 12 |
| Location: | 6508950 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g538650 | UPM3,SUMT3,COBA,HEM4 | S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase; (1 of 1) 2.1.1.107 - Uroporphyrinogen-III C-methyltransferase / Uroporphyrinogen-III methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATCGGGAACCGGTGTGTGGGCGTGTCAA |
| Internal bar code: | TTGACCGGTTTGTTGTACGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 99.6815 |
| LEAP-Seq n confirming: | 8762 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGGGGGACTACATTCAT |
| Suggested primer 2: | ACTTACAGGTGTATCGGGCG |