| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.217718 |
| Chromosome: | chromosome 10 |
| Location: | 5942254 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462250 | REF1 | RNA Export Factor; (1 of 1) K12881 - THO complex subunit 4 (THOC4, ALY) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAACAGGCGAAGCGCGCTTGAGAACACAG |
| Internal bar code: | TTGCTCAAAGGCTTTTCTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 199 |
| LEAP-Seq percent confirming: | 1.16279 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 5270 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCCATGGAGATTCTGTT |
| Suggested primer 2: | GCCCTTACCACTGGACTTCA |