| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.217853 |
| Chromosome: | chromosome 10 |
| Location: | 2399599 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435400 | (1 of 1) PF03398 - Regulator of Vps4 activity in the MVB pathway (Ist1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGCAACAAGAAACAGGTCCAGGTGAGTG |
| Internal bar code: | AGGCGAAGTCGAGGGAACATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 99.3301 |
| LEAP-Seq n confirming: | 7414 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTTCTGGAGGTAGGGG |
| Suggested primer 2: | CGGTTTAGTTTGGGCTGGTA |