| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.217875 |
| Chromosome: | chromosome 6 |
| Location: | 5346714 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283500 | POB3 | Proteome of basal body 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAACGCCTTGCGGAAGCCCCTGTAAGCC |
| Internal bar code: | CGTCGACGTTGCCCCGCGAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 99.4813 |
| LEAP-Seq n confirming: | 3452 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAACCTTCAGAGAACAGCC |
| Suggested primer 2: | GCCCTTATCTGGGCCTATTC |