| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218092 |
| Chromosome: | chromosome 12 |
| Location: | 3418417 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496700 | TRX17,TRL3 | (1 of 4) PTHR10438//PTHR10438:SF255 - THIOREDOXIN // THIOREDOXIN M4, CHLOROPLASTIC; Thioredoxin-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGAGTGGTCCCCCGTCAGCTCCAGCAGC |
| Internal bar code: | ACGATCAGACAATTTTGTCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1008 |
| LEAP-Seq percent confirming: | 99.5048 |
| LEAP-Seq n confirming: | 3617 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGTTCAAGCAAATGGGC |
| Suggested primer 2: | ATTTGACGCTTAAACGACCG |