Insertion junction: LMJ.RY0402.218220_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.218220
Chromosome:chromosome 13
Location:2469963
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g580000 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGATATAGCCTTGCCGCTTGCCGCGTCTC
Internal bar code:TGCGCTAACGGGGCGTCGGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:873
LEAP-Seq percent confirming:99.9093
LEAP-Seq n confirming:2202
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTCTGCTGTCTTCTAGGG
Suggested primer 2:CTCCCTCTGCTGCATGTGTA