| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218338 |
| Chromosome: | chromosome 12 |
| Location: | 3089623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500200 | MPA2 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR22953:SF18 - METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGTTGGTGTCGCGGCAGGGGCCGGCCT |
| Internal bar code: | AAAAGCTTACAGTTGGCACTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 630 |
| LEAP-Seq percent confirming: | 99.5592 |
| LEAP-Seq n confirming: | 4517 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGCGCTTCTCATTCATT |
| Suggested primer 2: | CTCGGAAGACAGCATCACAA |