| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218529 |
| Chromosome: | chromosome 1 |
| Location: | 5484535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038650 | SRS2 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 2) K12900 - FUS-interacting serine-arginine-rich protein 1 (FUSIP1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACGACGGCACGATGACGAAGAGCTGCTTT |
| Internal bar code: | CACTACCGGTAGGCTGGTTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 99.7141 |
| LEAP-Seq n confirming: | 2093 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACACAGCGGCATAGCTACA |
| Suggested primer 2: | CAGTGTTTGAGAGGCAAGCA |