| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218540 |
| Chromosome: | chromosome 10 |
| Location: | 2674974 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437829 | CIA6,CGL89 | (1 of 1) PTHR13271//PTHR13271:SF19 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // PROTEIN SET DOMAIN GROUP 40; methyltransferase domain protein involved in pyrenoid biogenesis | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAAGGCATGAGAGAGAACCGCTGAGTC |
| Internal bar code: | ATCGACCGTGTACAGCCGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 300 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTGAGGACCTGCCTAC |
| Suggested primer 2: | ATTGCAGCAAAGCCAGTTCT |