| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219155 |
| Chromosome: | chromosome 3 |
| Location: | 8330448 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g198950 | CGL30 | (1 of 3) PTHR31407//PTHR31407:SF18 - FAMILY NOT NAMED // PSBP DOMAIN-CONTAINING PROTEIN 4, CHLOROPLASTIC; Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAGTTTGACTACCTGCTGTGCACCTTG |
| Internal bar code: | AGCAAGCAAGTGGAACTCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 796 |
| LEAP-Seq percent confirming: | 99.106 |
| LEAP-Seq n confirming: | 6984 |
| LEAP-Seq n nonconfirming: | 63 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCTGCGGGTCATAGGTG |
| Suggested primer 2: | TGCGAGAAATGATGATGAGC |