| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219441 |
| Chromosome: | chromosome_9 |
| Location: | 561035 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g403800 | DYHB,ROC30,ROC54,ODA4 | Flagellar outer arm dynein heavy chain beta, involved in circadi | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATGCCCTCAGCCATCAGCATAATCTCGCA |
| Internal bar code: | GGCTAGACTGGGACGTGCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 879 |
| LEAP-Seq percent confirming: | 99.7836 |
| LEAP-Seq n confirming: | 5533 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGTGCAGGAGAGGTCAGG |
| Suggested primer 2: | TGGTGCAGAAGCTGATTGAC |