| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219443 |
| Chromosome: | chromosome 9 |
| Location: | 2131579 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393551 | (1 of 1) IPR001202//IPR002713 - WW domain // FF domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCCAGCACCCGGCGCGCAGTGTTTGTTG |
| Internal bar code: | CACTATTTTAAGTATCTTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 99.5381 |
| LEAP-Seq n confirming: | 3017 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCCAAACTAAACCAAA |
| Suggested primer 2: | GCCGGAGGACAGAGACATAG |