| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219478 |
| Chromosome: | chromosome 3 |
| Location: | 6044481 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g191250 | LCI34 | Low-CO2-inducible protein 34 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAAACCGCGCCCGAATCCCCATCGACCA |
| Internal bar code: | GAGAAGGTTGGCGAGCTGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 99.4609 |
| LEAP-Seq n confirming: | 738 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCCATACACCATGACAC |
| Suggested primer 2: | TATGTGCAGCAGCCAGTAGG |