| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219596 |
| Chromosome: | chromosome 5 |
| Location: | 674271 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g230850 | (1 of 1) IPR001374//IPR002110//IPR020683 - R3H domain // Ankyrin repeat // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCAGAAAGCTGCTGGTGCCGTCAAGACG |
| Internal bar code: | TGGCCTCCGACTCCATATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 99.4783 |
| LEAP-Seq n confirming: | 5911 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCACCGATGCCTACAGGT |
| Suggested primer 2: | TGGGATTCGGCTCTTGTATC |