| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219787 |
| Chromosome: | chromosome_14 |
| Location: | 962679 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613950 | ABC2 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCGATTCCTGGCGACAGCGCTCACAGGTGG |
| Internal bar code: | CGGCGGCGCTTATCGGGCGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1102 |
| LEAP-Seq percent confirming: | 99.7267 |
| LEAP-Seq n confirming: | 4743 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCTTCTGAACTAAGTCG |
| Suggested primer 2: | TGAAGGTCACGACTCTCACG |