Insertion junction: LMJ.RY0402.219787_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219787
Chromosome:chromosome 14
Location:962679
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g613950 ABCA2,ABC2 (1 of 2) 3.6.3.32 - Quaternary-amine-transporting ATPase; ABC lipid exporter 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGATTCCTGGCGACAGCGCTCACAGGTGG
Internal bar code:CGGCGGCGCTTATCGGGCGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1102
LEAP-Seq percent confirming:99.7267
LEAP-Seq n confirming:4743
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CCGCCTTCTGAACTAAGTCG
Suggested primer 2:TGAAGGTCACGACTCTCACG