Insertion junction: LMJ.RY0402.220077_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.220077
Chromosome:chromosome 4
Location:1924265
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g218150 PPH1,PPP18 Thylakoid phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGGGGAATAATGATTACAGCTAGGACAA
Internal bar code:CAACCAAGGGGATGGTGTATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1125
LEAP-Seq percent confirming:98.8791
LEAP-Seq n confirming:8998
LEAP-Seq n nonconfirming:102
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGAAATTAGCAAGCGTCC
Suggested primer 2:ACTTAACCCTCCCACGCTTT