| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.220400 |
| Chromosome: | chromosome 1 |
| Location: | 1848827 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010050 | (1 of 18) 3.5.1.98 - Histone deacetylase / HDAC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAGATGCAACAGCACATGCAACAGCAC |
| Internal bar code: | GTGAGGGCACGTATGCACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 76.0841 |
| LEAP-Seq n confirming: | 1737 |
| LEAP-Seq n nonconfirming: | 546 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTAACCGTGTGCTTCAC |
| Suggested primer 2: | AGCGACTCCGTAGACACGTT |