| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.220543 |
| Chromosome: | chromosome_14 |
| Location: | 968989 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre14.g613950 | ABC2 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCCTTGCTGGTGTCATCATTTCCCCTCC |
| Internal bar code: | GAGCCGGGGAGTTGGCGGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.8671 |
| LEAP-Seq n confirming: | 3758 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGTCGTACTGGTGTGG |
| Suggested primer 2: | ACTTCACTCCCCTCCACCTC |