| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.220745 |
| Chromosome: | chromosome 2 |
| Location: | 5555852 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108750 | (1 of 6) PTHR23257//PTHR23257:SF529 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGTCCCTGTTGACCCGGCCCTACCTGC |
| Internal bar code: | TGGAAAGAATTCGGCCTATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1206 |
| LEAP-Seq percent confirming: | 99.326 |
| LEAP-Seq n confirming: | 7074 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGTGTTACTGTCCCGTT |
| Suggested primer 2: | TACAATCGCAAACCCTCCTC |