| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.221449 |
| Chromosome: | chromosome 1 |
| Location: | 7499846 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053950 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACGGAGAGCAGCCCCGCCGCCGCCGACA |
| Internal bar code: | GAGCGACTTGAATTGCAAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 277 |
| LEAP-Seq percent confirming: | 97.6744 |
| LEAP-Seq n confirming: | 252 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGATGAGCACGACTGGA |
| Suggested primer 2: | GGTCTTACCAGCAGCGTCTT |