| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.221562 |
| Chromosome: | chromosome_3 |
| Location: | 3468516 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g167550 | POC3,CEP290 | Centriole proteome protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTGCTGCAATGCCCCAGCCTCACACGCCA |
| Internal bar code: | CTGTCAACACGTCGCCGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 178 |
| LEAP-Seq percent confirming: | 77.496 |
| LEAP-Seq n confirming: | 489 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTATATGTGCGCAGCTGGA |
| Suggested primer 2: | CATCTATAGAAGGAGCGGCG |