Insertion junction: LMJ.RY0402.221976_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.221976
Chromosome:chromosome 3
Location:2109709
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g156900 LHCBM5 (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATGCTTTCGTAACCCCAACAGGACAAGC
Internal bar code:GTTTGCCGGTCCAGGAGTGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:974
LEAP-Seq percent confirming:99.5666
LEAP-Seq n confirming:22055
LEAP-Seq n nonconfirming:96
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCCATCCCTCCCTCTCTC
Suggested primer 2:CATGGTTTCTGCATTTCCCT