| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.221976 |
| Chromosome: | chromosome 3 |
| Location: | 2109714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g156900 | LHCBM5 | (1 of 6) K08912 - light-harvesting complex II chlorophyll a/b binding protein 1 (LHCB1); Light-harvesting Chlorophyll a/b binding protein of LHCII | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATTCAGGTTAGCAACAAGCTATATCATTC |
| Internal bar code: | GACGATGGAGGGCGCCCATCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1047 |
| LEAP-Seq percent confirming: | 99.7408 |
| LEAP-Seq n confirming: | 6926 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCATCCCTCCCTCTCTC |
| Suggested primer 2: | CATGGTTTCTGCATTTCCCT |