Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.221989 |
Chromosome: | chromosome_12 |
Location: | 8962169 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g544114 | CYN20-6 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CCAAGTTGTGCGGGTGCTATGTTGGGGTAT |
Internal bar code: | CGTCACACGATACTGATGGCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 647 |
LEAP-Seq percent confirming: | 99.7415 |
LEAP-Seq n confirming: | 5016 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGAAGAAGAATGGCCTTG |
Suggested primer 2: | GCTGTAACCGCCAGTACCAT |