| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222209 |
| Chromosome: | chromosome 16 |
| Location: | 1159852 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650450 | FAP219 | Flagellar Associated Protein 219 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCAAAGCAGCGCTCATGCGCCACACC |
| Internal bar code: | CGATAGCCGGAGCTGAACGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 99.5092 |
| LEAP-Seq n confirming: | 10543 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTTGGTGGTGTAGCCCTG |
| Suggested primer 2: | CACTCAGCCCACAACTCAGA |