| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222350 |
| Chromosome: | chromosome 1 |
| Location: | 5088183 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035350 | (1 of 1) K07512 - mitochondrial trans-2-enoyl-CoA reductase (MECR, NRBF1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTGGCGGCGGCCGCCAGCCCGATGTCT |
| Internal bar code: | AACCATCGGTTCGGGGGTCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 25.2101 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 89 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGAGGTCCTCCTTGAGC |
| Suggested primer 2: | AGCCGTACAGACCTTGCTGT |