| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222720 |
| Chromosome: | chromosome 9 |
| Location: | 4133469 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393765 | LMR1 | (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGAACACTGCATCTACTGCACACGCCC |
| Internal bar code: | TGACTCAGGGGCTAGCCAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 633 |
| LEAP-Seq percent confirming: | 99.6576 |
| LEAP-Seq n confirming: | 5239 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCCGCGCTAGATATTC |
| Suggested primer 2: | GATTGGAGCAGAGATCGAGG |