Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.222959 |
Chromosome: | chromosome_14 |
Location: | 476369 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre14.g611100 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAGGGGTGGAGGGTTGAAGGCAGTACACGA |
Internal bar code: | GTCTAATCCGTCCGATGTACAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 404 |
LEAP-Seq percent confirming: | 99.8402 |
LEAP-Seq n confirming: | 3123 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACATAACCACATTGCCAGC |
Suggested primer 2: | GGGTACCGCAGTCACTTGAT |