| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.222966 |
| Chromosome: | chromosome 11 |
| Location: | 685015 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467622 | (1 of 1) 1.1.1.317 - Perakine reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTATGTACGGCCAATGCATGATGGCGA |
| Internal bar code: | GGATTCACGCACAGACCGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 426 |
| LEAP-Seq percent confirming: | 98.3201 |
| LEAP-Seq n confirming: | 2107 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGATGGCAACGATGATGT |
| Suggested primer 2: | GCGAGAACTTTTTGACCAGC |