| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.223125 |
| Chromosome: | chromosome 3 |
| Location: | 4558685 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176833 | NAP1,NAP,DII5 | (1 of 7) PF00022 - Actin (Actin); Novel actin protein 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGGAGACGTTCCGTGTGCCCGCCATG |
| Internal bar code: | CTGAGGGCCAGGTGTTACTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 98.239 |
| LEAP-Seq n confirming: | 2622 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCAGCACAATCCCTGAAG |
| Suggested primer 2: | GTTGAGAGGCTGCCGACTAC |