| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223129 |
| Chromosome: | chromosome 17 |
| Location: | 396039 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698750 | RAA12,OPR104 | psaA mRNA trans-splicing factor of subcomplex I; (1 of 781) IPR000104 - Antifreeze protein, type I | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTATACTAACATACTCACCTGAGCTCAAA |
| Internal bar code: | GCTGGACGCCACGGCCCGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 242 |
| LEAP-Seq percent confirming: | 95.5556 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGGTGTCTGTGCTGTTG |
| Suggested primer 2: | CGTTCTGCTACACTCGCTCA |