| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.223150 |
| Chromosome: | chromosome 9 |
| Location: | 7805506 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416400 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCCCTGGCCGCCCTGCCCGATCTGTTTC |
| Internal bar code: | GCAAGTTCCGAGTCACAAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 821 |
| LEAP-Seq percent confirming: | 97.3468 |
| LEAP-Seq n confirming: | 1541 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGACCTCATTAGCCACGTC |
| Suggested primer 2: | TGATGCCTGTCTGCTGGTAG |