Insertion junction: LMJ.RY0402.223571_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.223571
Chromosome:chromosome 1
Location:7577203
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g054700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCCTGGCGACCGCAACGCGCAGCCGCTC
Internal bar code:CGGTGGCAACTCGCCGGGCTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:614
LEAP-Seq percent confirming:97.8709
LEAP-Seq n confirming:2942
LEAP-Seq n nonconfirming:64
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:AAATCCAATGACGAAGACGG
Suggested primer 2:AACTGTTATTTTGGCGTCCG