| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.224080 |
| Chromosome: | chromosome 2 |
| Location: | 3380118 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095102 | VPS5C | (1 of 4) PTHR10555:SF84 - SORTING NEXIN-8; Subunit of Retromer complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGCGTGACCAGGGCTGCCCACAAACC |
| Internal bar code: | ACCCCCAAGTCTCGGTCAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 80 |
| LEAP-Seq percent confirming: | 99.4671 |
| LEAP-Seq n confirming: | 560 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGAGCTTTATGGAGGAGGA |
| Suggested primer 2: | GGAGAAGCAGGAATGCTGAC |