Insertion junction: LMJ.RY0402.224260_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.224260
Chromosome:chromosome 4
Location:675192
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g217951 LCI33 Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGGGTGCGAGCCCAGAGCAAATATACT
Internal bar code:ACGTCCTGAGGGACGAGTGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:432
LEAP-Seq percent confirming:93.8949
LEAP-Seq n confirming:2430
LEAP-Seq n nonconfirming:158
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTCAAAGCTAACCGCGTC
Suggested primer 2:TTTGCAACAAGCTCATCGAC